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Although rare, alpha-lipoic acid may cause insulin autoimmunity (see Concerns and Cautions)

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Samples were diluted 1:4 with AA, of which 2 µl were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using the LC-MS-8060 system (Shimadzu) with a flow rate of 0.2 ml/min and following gradient: 0-10 min: 1-16% B, 10.01-15.00 min: 1% B (A being 0.1% formic acid